In-Country Activity Implementation Plan in Vietnam for JFY2006 (英文のみ) |
2. Epidemiological survey on bovine trypanosomiasis (Trypanosoma evansi infection)
|
Background
Trypanosoma evansi is not normally very pathogenic to animals and most of the infected animals remain asymptomatic. However, the pathogenicity seems to depend on the region, namely, the parasite strain prevalent in the region, and the animals newly introduced into such a region are likely to suffer from clinical trypanosomiasis. In the northern Vietnam, the cattle brought into the coastal areas from mountain regions often develop severe clinical symptoms caused by T. evansi and a number of them take even fatal courses. Therefore, the prevalence of the parasite should be elucidated and the prevalent strains genetically characterized to control the disease in the areas. |
Purpose
1. To investigate the prevalence of T. evansi in beef cattle in north Vietnam
2. To study the genetic characteristics of the parasites isolated through the sampling
3. To familiarize the center staff with serological and genetic examinations so as to become able to set up the system for other diseases and to cope with the difficulties encountered
|
Methods
1. Sampling
Sites: 3 provinces in the north
1. Lang Son
2. Cao Bang
3. Ha Giang
Methods: Samplings are carried out with 5 center staff on three-day-trip basis for 2 times per site (2 sites x 3 times = 6 three-day trips in total). One hundred cattle will be sampled during each sampling (100 heads/sampling x 6 times = 600 cattle in total).
|
2. Test Methods
In the field, all the blood samples collected are examined by hematocrit centrifuge technique (Woo's test) as soon as after sampling and positive samples inoculated into mice for isolation. In the laboratory, sera are utilized for antibody- and, if available, antigen-detection ELISA. The mice inoculated with infected blood samples are monitored for parasitemia and isolated T. evansi strains further tested genetically by PCR.
|
Input
1. Japanese expert (long-term expert: antigen-detection ELISA if possble)
2. Thai expert (antibody-detection ELISA, PCR)
3. Necessities for sampling and laboratory examination, daily allowance, accommodation, fuel cost, etc.
4. Equipment for JFY 200: ELISA plate washer
|
Expected Results
1. The prevalence and pathogenicity of T. evansi will be elucidated in the study areas, which can be utilized for the development of control measures against trypanosomiasis.
2. The staff will become able to apply the same technique for the diagnosis of other diseases through the establishment of ELISA (not a commercial kit), which results in the improvement of the diagnostic capability of the center.
|
Personnel in Charge
Dr. Vu Dang Dong Chief, Parasitology Section, NCVD |