| Specimen |
Objectives |
Remarks |
| 1. |
Tissue |
| |
- Carcass |
| |
- Organs |
| |
- Lesion |
| |
- Aborted fetus |
| |
- Stillborn |
|
| - isolation |
| - toxicology |
| - histopathology |
| - antigen |
|
| Isolation Specimen : |
| - use sterile equipment |
| - collect in sterile container separately |
| - label properly |
- quick shipment to the laboratory with adequate transportation
medium and refrigeration |
| Histopathological Specimen : |
| - prepare in smaller tissue blocks (0.5 cm thickness) |
- place in 10% buffered formalin, (10 times the volume of the
tissue sample) |
| - do not freeze |
|
| 2. |
Blood |
| |
- Whole blood |
| |
- Blood smear |
| |
- Serum |
| |
- Plasma |
|
| - hematology |
| - culture |
| - direct exam. |
| - serology |
|
| Specimen : |
| - collect as cleanly as possible |
| Whole Blood : |
| - add anti-coagulants (such as heparin or EDTA) and mix well |
| - keep in cool condition, do not freeze |
| Blood smear : |
- smear on a microscope slide, air dry and fix with absolute
methanol |
| Serum Specimen : |
| - allow blood to clot at ambient temperature (about 1 hour) |
- place the clotted blood in refrigerator (4ﾟC) or cool condition
during tansportation or overnight |
| - centrifuge and removed the clotted blood, keep the serum |
| - serum should be kept in frozen condition |
|
|
|
| - parasitology |
| - virology |
| - bacteriology |
|
| Feces Specimen : |
| - freshly voided |
| - send with or without transportation medium |
- place in containers to reduce air and prevent hatching of
parasite eggs |
| - keep in cool condition |
|
|
|
|
| Milk Specimen : |
| - use sterile equipment |
| - collect after cleaning the teat and discarding the intial stream |
| - collect at least 10 ml |
| - keep in cool condition |
| - quick shipment to the laboratory |
|
|
|
| - bacteriology |
| - parasitology |
| - urine analysis |
|
| Specimen : |
| - keep in sterile containers |
| - keep in cool condition |
|
|
|
| - bacteriology |
| - parasitology |
|
| Specimen : |
| - vaginal or preputial washing |
| - cervical or urethral swab |
| - keep in cool condition |
|
|
|
| - bacteriology |
| - virology |
| - parasitology |
|
| Specimen : |
| - scrap the lesion |
| - take the vesicular fluid |
| - keep in cool condition |
|
|
|
|
| Specimen : |
| - collect by cotton swabs wetted with transport medium |
| - ship to the laboratory at 4ﾟC |
|
|
|
|
| Internal Parasites : |
| - keep in closed containers |
| - fix in 10% buffered formalin |
| External Parasites : |
| - fix in 10% buffered formalin or 70% alcohol |
|
| 10. |
Environmental |
| |
Specimens : |
| |
- Feed |
| |
- Water |
| |
- Soil |
|
| - hygiene |
| - monitoring |
| - feed analysis |
|
| Dry Specimen : |
| - keep in clean and dry containers with moisture protection |
| Fresh Specimen : |
| - keep in cool condition |
|
