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45. Haemophilus parasuis infection

Causative agent
- Haemophilus parasuis


Differential diagnosis
- Swine fever
- Aujeszky's disease
- Swine influenza
- Streptococcosis
- Pleuropneumonia
- Pasteurellosis
- Swine erysipelas
- Mycoplasmal pneumonia of swine
- Toxoplasmosis


1. History taking
- Sporadic and limited to one farm with a variable
  rate of morbidity and mortality
- Stress by environment, feeding and shipping
- Usually occur in 3 to 8 weeks age piglets,
  especially in SPF farm


2. Clinical examination
- Depression
- Moderate to high fever
- Vomit
- Nervous symptoms
- Arthritis


3. Necropsy
- Turbidity and increasing of fibrino-purulent
  meningitis
- Fibrinous pleurisy
- Pericarditis and increase of pericardial fluid
- Hydrothorax
- Ascitis and peritonitis
- Increase of fibrin on synovial membrane and
  turbidity of the fluid


4. Histopathological observation
- Serofibrinous suppurative meningitis
- Serofibrinous, suppurative arthritis and fibrinous
  synovitis
- Serofibrihous pericarditis, pleuritis and peritonitis


5. Bacterial isolation
Specimen : visceral organs and synovial fluid
Medium    : blood agar
- Culture  : incubate the agar at 37°C for 24 hr, under
  5% C02 condition
- Gram negative and pleomorphic rod of various length,
  transparent satellite colonies grown around the
  Staphylococcus inoculated line


Table: Biochemical characters of H. parasuis
Porphyrin CO2 V-factor Urease Hemolysis
+ + + - -


6. Serotyping
- AGID test


Control
- Avoid exposing to stress condition
- Prophylactic dosing of antibiotic at the time of
  shipping or medication of feed or drinking water
  on arrival
- Formalinized-killed bacterin administered before
  weaning



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