Causative agent
- Trypanosoma evansi
Differential diagnosis
- Babesiosis
- Anaplasmosis
- Theileriosis
1. History taking
- Susceptible in all domestic animals, fatal disease can occur in horses and dogs
- Less pathogenic infection in buffaloes, cattle and pigs
- Decreased milk production and occasionally causing abortion
- Vectors are horse fly (Tabanus sp.) and stable fly (Stomoxy sp.)
- Outbreak often occurs after rainy season
2. Clinical examination
- Anorexia emaciation
- Anemia to jaundice
- Stiffness and swelling of legs
- Intermittent fever
- Opacity of the cornea
- Late abortion (6-7 months)
- infected cattle may show nervous signs such
as cycling and convulsion
3. Microscopic examination
- Demonstration of trypanosomes from wet blood film, thick and thin blood smears, and cerebrospinal fluid
- Trypanosomes are best demonstrated in early infections and in acute desease
- Hematocrit centrifuge technique (HCT)
- Quantitative buffy coat technique (QBC)
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4. Experimental animal inoculation
- Inoculate suspected blood into laboratory rodents
5. Serological tests
- Card agglutination test (CATT)
- IFAT
- ELISA
6. Necropsy
- Patchy alopecia
- Petechiae and ecchymoses of visible mucosa
- Edema of the limbs, lower abdomen and thorax
- lcterus
7. Histopathological observation
- Detection of Trypanosoma in brain, lung, spleen and liver
Control and treatment
- Preventive control with trypanocidal drugs before and after fly season
- Fly control by regular application with effective insecticides to animals
- Whole herd treatment with trypanocidal drugs such as isometamidium chloride (SamorinR), or diminazene aceturate (BerenilR) on the positive farm
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