5) Colonies of Streptococcus agalactiae (blood agar, 37 C for 48 hrs).
6) Bacteria of Staphylococcus aureus (Giemsa staining).
7) Bacteria of Actinomyces pyogenes (Giemsa staining).
8) Determination of N-acetyl-B-B-glucosa-minidase activity in milk sample (from left); mastitis negative to doutful, then degree of infection expressing + to +++